Special Section on Tissue and Blood Optical Clearing for Biomedical Applications

Optical clearing of skin enhanced with hyaluronic acid for increased contrast of optoacoustic imaging

[+] Author Affiliations
Anton Liopo, Dmitri A. Tsyboulski

TomoWave Laboratories, 6550 Mapleridge Street Suite 124, Houston, Texas 77081, United States

Richard Su, Alexander A. Oraevsky

TomoWave Laboratories, 6550 Mapleridge Street Suite 124, Houston, Texas 77081, United States

University of Houston, Department of Biomedical Engineering, 3600 Calhoun Road, Houston, Texas 77004, United States

J. Biomed. Opt. 21(8), 081208 (May 27, 2016). doi:10.1117/1.JBO.21.8.081208
History: Received January 27, 2016; Accepted April 18, 2016
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Abstract.  Enhanced delivery of optical clearing agents (OCA) through skin may improve sensitivity of optical and optoacoustic (OA) methods of imaging, sensing, and monitoring. This report describes a two-step method for enhancement of light penetration through skin. Here, we demonstrate that topical application of hyaluronic acid (HA) improves skin penetration of hydrophilic and lipophilic OCA and thus enhances their performance. We examined the OC effect of 100% polyethylene and polypropylene glycols (PPGs) and their mixture after pretreatment by HA, and demonstrated significant increase in efficiency of light penetration through skin. Increased light transmission resulted in a significant increase of OA image contrast in vitro. Topical pretreatment of skin for about 30 min with 0.5% HA in aqueous solution offers effective delivery of low molecular weight OCA such as a mixture of PPG-425 and polyethylene glycol (PEG)-400. The developed approach of pretreatment by HA prior to application of clearing agents (PEG and PPG) resulted in a 47-fold increase in transmission of red and near-infrared light and significantly enhanced contrast of OA images.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Anton Liopo ; Richard Su ; Dmitri A. Tsyboulski and Alexander A. Oraevsky
"Optical clearing of skin enhanced with hyaluronic acid for increased contrast of optoacoustic imaging", J. Biomed. Opt. 21(8), 081208 (May 27, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.081208


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