Research Papers: General

In vivo/ex vivo targeting of Langerhans cells after topical application of the immune response modifier TMX-202: confocal Raman microscopy and histology analysis

[+] Author Affiliations
Maxim E. Darvin, Gisela Thiede, Sabine Schanzer, Heike Richter, Juergen Lademann

Charité—Universitätsmedizin Berlin, Department of Dermatology, Venerology, and Allergology, Charitéplatz 1, 10117 Berlin, Germany

Saul Mujica Ascencio

Charité—Universitätsmedizin Berlin, Department of Dermatology, Venerology, and Allergology, Charitéplatz 1, 10117 Berlin, Germany

Centro de Investigación e Innovación Tecnológica (CIITEC) del Instituto Politécnico Nacional (IPN), Cerrada de Cecati S/N, Col. Santa Catarina, Azcapotzalco, CP: 02250 México D. F., Mexico

Sabrina E. Vinzón, Daniel Hasche, Frank Rösl

DKFZ—Division of Viral Transformation Mechanisms, German Cancer Research Center, Im Neuenheimer Feld 280, Heidelberg 69120, Germany

Roberto May

Telormedix S.A, Via Lugano 13, Agno CH-6982, Switzerland

Yohan Hazot, Dov Tamarkin

Foamix Pharmaceuticals Ltd., 2 Holzman Street, Weizmann-Science Park, Rehovot 7670402, Israel

J. Biomed. Opt. 21(5), 055004 (May 27, 2016). doi:10.1117/1.JBO.21.5.055004
History: Received December 21, 2015; Accepted May 3, 2016
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Abstract.  The increased ability of TMX-202 (derivative of imiquimod) to penetrate the intact stratum corneum (SC) and the follicular orifices of porcine ear skin was shown ex vivo using confocal Raman microscopy and laser scanning microscopy. Moreover, to assess whether TMX-202 is able to reach the immune cells, Langerhans cells extracted from pretreated human skin were investigated ex vivo using confocal Raman microscopy combined with multivariate statistical methods. Tracking the Raman peak of dimethyl sulfoxide centered at 690  cm1, the absorption of TMX-202 containing formulation by Langerhans cells was shown. To answer the question whether the TMX-202 active ingredient is able to reach Langerhans cells, the attraction of immune cells to TMX-202 containing formulation treated skin was measured in the in vivo rodent model Mastomys coucha. The results show that TMX-202 active ingredient is able to reach Langerhans cells after penetrating through the intact skin and subsequently attract immune cells. Both the intercellular/transcellular as well as the follicular pathways allow the penetration through the intact barrier of the SC.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Maxim E. Darvin ; Gisela Thiede ; Saul Mujica Ascencio ; Sabine Schanzer ; Heike Richter, et al.
"In vivo/ex vivo targeting of Langerhans cells after topical application of the immune response modifier TMX-202: confocal Raman microscopy and histology analysis", J. Biomed. Opt. 21(5), 055004 (May 27, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.5.055004


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