Research Papers: Imaging

Photoacoustic molecular imaging for in vivo liver iron quantitation

[+] Author Affiliations
Federica Maccarinelli, Fernando Carmona, Maria Regoni, Paolo Arosio

University of Brescia, Department of Molecular and Translational Medicine, Laboratory of Molecular Biology, Viale Europa 11, Brescia 25123, Italy

J. Biomed. Opt. 21(5), 056008 (May 27, 2016). doi:10.1117/1.JBO.21.5.056008
History: Received March 1, 2016; Accepted May 11, 2016
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Abstract.  A recent study showed that ferritin is a suitable endogenous contrast agent for photoacoustic molecular imaging in cultured mammalian cells. We have therefore tested whether this imaging technique can be used for in vivo quantification of iron in mouse livers. To verify this hypothesis, we used multispectral optoacoustic tomography (MSOT) to image albino CD1 mice before and after experimental iron loading. Postmortem assays showed that the iron treatment caused a 15-fold increase in liver iron and a 40-fold increase in liver ferritin levels, while in vivo longitudinal analysis using MSOT revealed just a 1.6-fold increase in the ferritin/iron photoacoustic signal in the same animals. We conclude that MSOT can monitor changes in ferritin/iron levels in vivo, but its sensitivity is much lower than that of ex vivo iron assays.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Federica Maccarinelli ; Fernando Carmona ; Maria Regoni and Paolo Arosio
"Photoacoustic molecular imaging for in vivo liver iron quantitation", J. Biomed. Opt. 21(5), 056008 (May 27, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.5.056008


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