JBO Letters

In vivo simultaneous multispectral fluorescence imaging with spectral multiplexed volume holographic imaging system

[+] Author Affiliations
Yanlu Lv, Jiulou Zhang, Dong Zhang, Wenjuan Cai

Tsinghua University, Department of Biomedical Engineering, Haidian District, Beijing 100084, China

Nanguang Chen

National University of Singapore, Department of Biomedical Engineering, Singapore 117576, Singapore

Jianwen Luo

Tsinghua University, Department of Biomedical Engineering, Haidian District, Beijing 100084, China

Tsinghua University, Center for Biomedical Imaging Research, Haidian District, Beijing 100084, China

J. Biomed. Opt. 21(6), 060502 (Jun 03, 2016). doi:10.1117/1.JBO.21.6.060502
History: Received March 2, 2016; Accepted May 9, 2016
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Abstract.  A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in the scene. The implementation and performance of the simultaneous multispectral imaging system are presented. Further, the system’s capability in simultaneously obtaining multispectral fluorescence measurements is demonstrated with in vivo experiments on a mouse. The demonstrated imaging system has the potential to obtain multispectral images fluorescence simultaneously.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Yanlu Lv ; Jiulou Zhang ; Dong Zhang ; Wenjuan Cai ; Nanguang Chen, et al.
"In vivo simultaneous multispectral fluorescence imaging with spectral multiplexed volume holographic imaging system", J. Biomed. Opt. 21(6), 060502 (Jun 03, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.6.060502


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