Research Papers: Imaging

Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image

[+] Author Affiliations
Mohammad Zaheer Ansari

Indian School of Mines, Department of Applied Physics, Biomedical Optics Laboratory, Dhanbad, Jharkhand 826004, India

Evelio E. Ramírez-Miquet, Isabel Otero, Dania Rodríguez, Juan G. Darias

Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear, Calle 30 No. 502, Miramar, C. P. 11300 La Habana, Cuba

J. Biomed. Opt. 21(6), 066006 (Jun 08, 2016). doi:10.1117/1.JBO.21.6.066006
History: Received February 19, 2016; Accepted May 17, 2016
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Abstract.  This paper reports the application of the motion history image (MHI) method for biospeckle processing of a bacterial growth. The method avoids the complexity as well as the large computation in sequence-matching-based methods and detects whether the speckle structure has changed or not. Encouraging experimental results on the real-time evolution of the growing bacteria during 12 h demonstrate the effectiveness of the proposed method. The MHI presented an online result without loss of resolution and definition. In turn, the MHI also presented the ability to provide a close answer to the traditional offline method of generalized differences.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Topics

Bacteria ; Lasers ; Speckle

Citation

Mohammad Zaheer Ansari ; Evelio E. Ramírez-Miquet ; Isabel Otero ; Dania Rodríguez and Juan G. Darias
"Real time and online dynamic speckle assessment of growing bacteria using the method of motion history image", J. Biomed. Opt. 21(6), 066006 (Jun 08, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.6.066006


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