Research Papers: General

Finite-difference time-domain-based optical microscopy simulation of dispersive media facilitates the development of optical imaging techniques

[+] Author Affiliations
Di Zhang, Ilker Capoglu, Lusik Cherkezyan, John Chandler, Hariharan Subramanian, Vadim Backman

Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States

Yue Li

Northwestern University, Applied Physics Program, 2145 Sheridan Road, Evanston, Illinois 60208, United States

Graham Spicer

Northwestern University, Department of Chemical and Biological Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States

Allen Taflove

Northwestern University, Department of Electrical Engineering and Computer Science, 2145 Sheridan Road, Evanston, Illinois 60208, United States

J. Biomed. Opt. 21(6), 065004 (Jun 10, 2016). doi:10.1117/1.JBO.21.6.065004
History: Received March 7, 2016; Accepted May 25, 2016
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Abstract.  Combining finite-difference time-domain (FDTD) methods and modeling of optical microscopy modalities, we previously developed an open-source software package called Angora, which is essentially a “microscope in a computer.” However, the samples being simulated were limited to nondispersive media. Since media dispersions are common in biological samples (such as cells with staining and metallic biomarkers), we have further developed a module in Angora to simulate samples having complicated dispersion properties, thereby allowing the synthesis of microscope images of most biological samples. We first describe a method to integrate media dispersion into FDTD, and we validate the corresponding Angora dispersion module by applying Mie theory, as well as by experimentally imaging gold microspheres. Then, we demonstrate how Angora can facilitate the development of optical imaging techniques with a case study.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Di Zhang ; Ilker Capoglu ; Yue Li ; Lusik Cherkezyan ; John Chandler, et al.
"Finite-difference time-domain-based optical microscopy simulation of dispersive media facilitates the development of optical imaging techniques", J. Biomed. Opt. 21(6), 065004 (Jun 10, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.6.065004


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