Special Section on Clinical Near-Infrared Spectroscopy and Imaging

Comprehensive phantom for interventional fluorescence molecular imaging

[+] Author Affiliations
Maria Anastasopoulou, Maximilian Koch, Dimitris Gorpas, Angelos Karlas, Pilar Beatriz Garcia-Allende, Vasilis Ntziachristos

Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstraße 1, Neuherberg D-85764 Germany

Technical University Munich, Chair for Biological Imaging, Arcisstraße 21, Munich D-80333, Germany

Uwe Klemm

Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstraße 1, Neuherberg D-85764 Germany

J. Biomed. Opt. 21(9), 091309 (Jun 15, 2016). doi:10.1117/1.JBO.21.9.091309
History: Received January 30, 2016; Accepted May 23, 2016
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Abstract.  Fluorescence imaging has been considered for over a half-century as a modality that could assist surgical guidance and visualization. The administration of fluorescent molecules with sensitivity to disease biomarkers and their imaging using a fluorescence camera can outline pathophysiological parameters of tissue invisible to the human eye during operation. The advent of fluorescent agents that target specific cellular responses and molecular pathways of disease has facilitated the intraoperative identification of cancer with improved sensitivity and specificity over nonspecific fluorescent dyes that only outline the vascular system and enhanced permeability effects. With these new abilities come unique requirements for developing phantoms to calibrate imaging systems and algorithms. We briefly review herein progress with fluorescence phantoms employed to validate fluorescence imaging systems and results. We identify current limitations and discuss the level of phantom complexity that may be required for developing a universal strategy for fluorescence imaging calibration. Finally, we present a phantom design that could be used as a tool for interlaboratory system performance evaluation.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Maria Anastasopoulou ; Maximilian Koch ; Dimitris Gorpas ; Angelos Karlas ; Uwe Klemm, et al.
"Comprehensive phantom for interventional fluorescence molecular imaging", J. Biomed. Opt. 21(9), 091309 (Jun 15, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.9.091309


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