Special Section on Tissue and Blood Optical Clearing for Biomedical Applications

Enhanced in vivo visualization of the microcirculation by topical application of fructose solution confirmed with correlation mapping optical coherence tomography

[+] Author Affiliations
Joey Enfield, James McGrath, Susan M. Daly

Tissue Optics and Microcirculation Imaging Facility, National Biophotonics and Imaging Platform, Department of Physics and Energy, Ireland

Martin Leahy

Tissue Optics and Microcirculation Imaging Facility, National Biophotonics and Imaging Platform, Department of Physics and Energy, Ireland

National University of Ireland, Tissue Optics and Microcirculation Imaging Group, National Biophotonics and Imaging Platform, School of Physics, Galway, Ireland

J. Biomed. Opt. 21(8), 081212 (Jun 17, 2016). doi:10.1117/1.JBO.21.8.081212
History: Received January 28, 2016; Accepted May 2, 2016
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Abstract.  Changes within the microcirculation can provide an early indication of the onset of a plethora of ailments. Various techniques have thus been developed that enable the study of microcirculatory irregularities. Correlation mapping optical coherence tomography (cmOCT) is a recently proposed technique, which enables mapping of vasculature networks at the capillary level in a noninvasive and noncontact manner. This technique is an extension of conventional optical coherence tomography (OCT) and is therefore likewise limited in the penetration depth of ballistic photons in biological media. Optical clearing has previously been demonstrated to enhance the penetration depth and the imaging capabilities of OCT. In order to enhance the achievable maximum imaging depth, we propose the use of optical clearing in conjunction with the cmOCT technique. We demonstrate in vivo a 13% increase in OCT penetration depth by topical application of a high-concentration fructose solution, thereby enabling the visualization of vessel features at deeper depths within the tissue.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Joey Enfield ; James McGrath ; Susan M. Daly and Martin Leahy
"Enhanced in vivo visualization of the microcirculation by topical application of fructose solution confirmed with correlation mapping optical coherence tomography", J. Biomed. Opt. 21(8), 081212 (Jun 17, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.081212


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