Research Papers: Imaging

Correlation of breast tissue histology and optical signatures to improve margin assessment techniques

[+] Author Affiliations
Stephanie Kennedy, Matthew Caldwell, Torre Bydlon, Christine Mulvey, Jenna Mueller, Nimmi Ramanujam

Duke University, TOpS Lab, Department of Biomedical Engineering, 136 Hudson Hall, Durham, North Carolina 27708, United States

Lee Wilke

University of Wisconsin Breast Center, Department of Surgery, 600 Highland Avenue, Madison WI 53792, United States

William Barry

Dana-Farber Cancer Institute, Department of Biostatistics and Computational Biology, 450 Brookline Avenue, CLS11007, Boston, Massachusetts 02215, United States

Joseph Geradts

Duke University Medical Center, Department of Pathology, DUMC3712, 200 Trent Drive, Durham, North Carolina 27710, United States

J. Biomed. Opt. 21(6), 066014 (Jun 21, 2016). doi:10.1117/1.JBO.21.6.066014
History: Received March 5, 2016; Accepted June 8, 2016
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Abstract.  Optical spectroscopy is sensitive to morphological composition and has potential applications in intraoperative margin assessment. Here, we evaluate ex vivo breast tissue and corresponding quantified hematoxylin & eosin images to correlate optical scattering signatures to tissue composition stratified by patient characteristics. Adipose sites (213) were characterized by their cell area and density. All other benign and malignant sites (181) were quantified using a grid method to determine composition. The relationships between mean reduced scattering coefficient (μs), and % adipose, % collagen, % glands, adipocyte cell area, and adipocyte density were investigated. These relationships were further stratified by age, menopausal status, body mass index (BMI), and breast density. We identified a positive correlation between μs and % collagen and a negative correlation between μs and age and BMI. Increased collagen corresponded to increased μs variability. In postmenopausal women, μs was similar regardless of fibroglandular content. Contributions from collagen and glands to μs were independent and equivalent in benign sites; glands showed a stronger positive correlation than collagen to μs in malignant sites. Our data suggest that scattering could differentiate highly scattering malignant from benign tissues in postmenopausal women. The relationship between scattering and tissue composition will support improved scattering models and technologies to enhance intraoperative optical margin assessment.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Stephanie Kennedy ; Matthew Caldwell ; Torre Bydlon ; Christine Mulvey ; Jenna Mueller, et al.
"Correlation of breast tissue histology and optical signatures to improve margin assessment techniques", J. Biomed. Opt. 21(6), 066014 (Jun 21, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.6.066014


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