Research Papers: General

Stimulus-evoked outer segment changes in rod photoreceptors

[+] Author Affiliations
Xiaohui Zhao

Hebei University, College of Physics Science and Technology, Hebei Key Lab of Optic-Electronic Information Materials, Baoding 071002, China

University of Illinois at Chicago, Department of Bioengineering, Chicago, Illinois 60607, United States

Damber Thapa, Benquan Wang, Yiming Lu, Shaoyan Gai

University of Illinois at Chicago, Department of Bioengineering, Chicago, Illinois 60607, United States

Xincheng Yao

Hebei University, College of Physics Science and Technology, Hebei Key Lab of Optic-Electronic Information Materials, Baoding 071002, China

University of Illinois at Chicago, Department of Bioengineering, Chicago, Illinois 60607, United States

University of Illinois at Chicago, Department of Ophthalmology and Visual Sciences, Chicago, Illinois 60612, United States

J. Biomed. Opt. 21(6), 065006 (Jun 23, 2016). doi:10.1117/1.JBO.21.6.065006
History: Received March 23, 2016; Accepted June 1, 2016
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Abstract.  Rod-dominated transient retinal phototropism (TRP) has been recently observed in freshly isolated mouse and frog retinas. Comparative confocal microscopy and optical coherence tomography revealed that the TRP was predominantly elicited from the rod outer segment (OS). However, the biophysical mechanism of rod OS dynamics is still unknown. Mouse and frog retinal slices, which displayed a cross-section of retinal photoreceptors and other functional layers, were used to test the effect of light stimulation on rod OSs. Time-lapse microscopy revealed stimulus-evoked conformational changes of rod OSs. In the center of the stimulated region, the length of the rod OS shrunk, while in the peripheral region, the rod OS swung toward the center region. Our experimental observation and theoretical analysis suggest that the TRP may reflect unbalanced rod disc-shape changes due to localized visible light stimulation.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Xiaohui Zhao ; Damber Thapa ; Benquan Wang ; Yiming Lu ; Shaoyan Gai, et al.
"Stimulus-evoked outer segment changes in rod photoreceptors", J. Biomed. Opt. 21(6), 065006 (Jun 23, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.6.065006


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