The two-color channels are spectrally separated using a dichroic (T650lpxr, Chroma Technology) and emission filters remove any additional background (Atto 647N: HQ660LP, HQ675/50M and HQ620/40M, Atto 590: HQ680/40M-HC, Chroma Technology) before coupling into two core multimode fibers (M31L01, Thorlabs Inc.). Each multimode fiber serves as both a confocal pinhole and couples the light to an avalanche photodiode (APD) detector (SPCM-AQRH-13-FC, Perkin Elmer, Inc.). The image is constructed using a high-resolution three-dimensional (3-D) piezoelectric stage that scans the sample with a maximum travel and travel (P-733.3DD, Physik Instrumente) with an analog/digital controller (E-725.3CDA, Physik Instrumente). A image with 10(20) nm pixels is acquired in 79(38) s. The stage is controlled from the computer’s data acquisition board (PCIe-6353, National Instruments, Inc.). The piezo stage is mounted to a motorized stage (MD5422LOU, Physik Instrumente) connected to a controller (C-867.260, Physik Instrumente) that scans the sample at lower resolutions with a joystick, for locating the region of the sample of interest for imaging. The user is able to align the sample using widefield epifluorescence, brightfield, and/or DIC imaging. For epifluorescence imaging, we use a light source (Lumen 200, Prior Scientific Inc.) that has higher emission at red wavelengths to visualize Atto 647N. A CCD camera (Axiocam MRm, Carl Zeiss Microscopy GmbH) on the right side port is used to visualize the fluorescence that is mostly outside visible range for far-red fluorophores.