Research Papers: General

Differentiating gastrointestinal stromal tumors from gastric adenocarcinomas and normal mucosae using confocal Raman microspectroscopy

[+] Author Affiliations
Chih-Wei Hsu

Tungs’ Taichung MetroHarbor Hospital, Division of General Surgery, Department of Surgery, No. 699, Section 8, Taiwan Boulevard, Wuqi, Taichung 43503, Taiwan

National Chiayi University, Department of Applied Chemistry, No. 300 Syuefu Road, Chiayi City 60004, Taiwan

Chia-Chi Huang

National Chung Cheng University, Center of Nano Bio-Detection, No. 168, Section 1, University Road, Min-Hsiung Township, Chia-yi County 621, Taiwan

Jeng-Horng Sheu, Wenlung Chen

National Chiayi University, Department of Applied Chemistry, No. 300 Syuefu Road, Chiayi City 60004, Taiwan

Chia-Wen Lin

HungKuang University, Department of Applied Cosmetology, No. 34, Zhongqi Road, Shalu Township, Taichung County, Taiwan

Lien-Fu Lin

Tungs’ Taichung MetroHarbor Hospital, Division of Gastroenterology, Department of Internal Medicine, No. 699, Section 8, Taiwan Boulevard, Wuqi, Taichung 43503, Taiwan

Jong-Shiaw Jin

Tungs’ Taichung MetroHarbor Hospital, Department of Pathology, No. 699, Section 8, Taiwan Boulevard, Wuqi, Taichung 43503, Taiwan

J. Biomed. Opt. 21(7), 075006 (Jul 11, 2016). doi:10.1117/1.JBO.21.7.075006
History: Received December 24, 2015; Accepted June 16, 2016
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Abstract.  Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms of the gastrointestinal tract, and gastric adenocarcinomas are a common cancer worldwide. To differentiate GISTs from adenocarcinomas is important because the surgical processes for both are different; the former excises the tumor with negative margins, while the latter requires radical gastrectomy with lymph node dissection. Endoscopy with biopsy is used to distinguish GISTs from adenocarcinomas; however, it may cause tumor bleeding in GISTs. We reported here the confocal Raman microspectroscopy as an effective tool to differentiate GISTs, adenocarcinomas, and normal mucosae. Of 119 patients enrolled in this study, 102 patients underwent gastrectomy (40 GISTs and 62 adenocarcinomas), and 17 patients with benign lesions were obtained as normal mucosae. Raman signals were integrated for 100 s for each spot on the specimen, and 5 to 10 spots, depending on the sample size, were chosen for each specimen. There were significant differences among those tissues as evidenced by different Raman signal responding to phospholipids and protein structures. The spectral data were further processed and analyzed by using principal component analysis. A two-dimensional plot demonstrated that GISTs, adenocarcinomas, and normal gastric mucosae could be effectively differentiated from each other.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Chih-Wei Hsu ; Chia-Chi Huang ; Jeng-Horng Sheu ; Chia-Wen Lin ; Lien-Fu Lin, et al.
"Differentiating gastrointestinal stromal tumors from gastric adenocarcinomas and normal mucosae using confocal Raman microspectroscopy", J. Biomed. Opt. 21(7), 075006 (Jul 11, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.7.075006


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