Research Papers: General

Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy

[+] Author Affiliations
Thiago de Oliveira Mendes, Liliane Pereira Pinto, Laurita dos Santos, Vamshi Krishna Tippavajhala, Claudio Alberto Téllez Soto, Airton Abrahão Martin

University of Vale do Paraíba, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, Av. Shishima Hifumi, 2911, Urbanova, CEP: 12.244-000, São José dos Campos, SP, Brazil

J. Biomed. Opt. 21(7), 075010 (Jul 13, 2016). doi:10.1117/1.JBO.21.7.075010
History: Received February 20, 2016; Accepted June 28, 2016
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Abstract.  The analysis of biological systems by spectroscopic techniques involves the evaluation of hundreds to thousands of variables. Hence, different statistical approaches are used to elucidate regions that discriminate classes of samples and to propose new vibrational markers for explaining various phenomena like disease monitoring, mechanisms of action of drugs, food, and so on. However, the technical statistics are not always widely discussed in applied sciences. In this context, this work presents a detailed discussion including the various steps necessary for proper statistical analysis. It includes univariate parametric and nonparametric tests, as well as multivariate unsupervised and supervised approaches. The main objective of this study is to promote proper understanding of the application of various statistical tools in these spectroscopic methods used for the analysis of biological samples. The discussion of these methods is performed on a set of in vivo confocal Raman spectra of human skin analysis that aims to identify skin aging markers. In the Appendix, a complete routine of data analysis is executed in a free software that can be used by the scientific community involved in these studies.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Thiago de Oliveira Mendes ; Liliane Pereira Pinto ; Laurita dos Santos ; Vamshi Krishna Tippavajhala ; Claudio Alberto Téllez Soto, et al.
"Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy", J. Biomed. Opt. 21(7), 075010 (Jul 13, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.7.075010


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