Research Papers: Imaging

Fluorescence Talbot microscope using incoherent source

[+] Author Affiliations
Yangyang Sun, Shuo Pang

University of Central Florida, Optical Imaging System Laboratory, The College of Optics and Photonics, 4304 Scorpius Street, Orlando, Florida 32816, United States

J. Biomed. Opt. 21(8), 086003 (Aug 08, 2016). doi:10.1117/1.JBO.21.8.086003
History: Received March 31, 2016; Accepted July 20, 2016
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Abstract.  Fluorescence Talbot microscope is a scalable field-of-view (FOV) imaging platform, which takes advantage of the phase sensitivity of the self-image of a periodic structure. Such a system can maintain the microscopic resolution and extend the FOV for the whole slide (15  mm×15  mm) scanning. Previously reported Talbot fluorescence systems, tabletop and on-chip device alike, rely on the coherence of the illumination source, limiting their potential applications in low-resource setting environment. A more cost-effective setup using a light-emitting diode, which has an area of 4  mm2 and a full width at half maximum of 16 nm in wavelength, is demonstrated. Compared to the illumination that is spatially filtered by a single pinhole, our system has achieved an illumination intensity that is 357 times higher. The reconstructed image quality is comparable to that of a 10× microscope objective. Various samples, such as fluorescent beads, green fluorescence protein-labeled HeLa cells, and a mouse kidney slide, were reconstructed by the system.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Yangyang Sun and Shuo Pang
"Fluorescence Talbot microscope using incoherent source", J. Biomed. Opt. 21(8), 086003 (Aug 08, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.086003


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