Research Papers: Sensing

Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection

[+] Author Affiliations
Min-Sheng Hung, Chih-Pin Chen

National Chiayi University, Department of Biomechatronic Engineering, No. 300 Syuefu Road, Chiayi 60004, Taiwan

Chia-Chin Ho

Chip Win Technology Co., Ltd., Biomedical and Advanced Systems Integration, No. 18 Sec. 2, Seng Yi Road, Zhubei City, Hsinchu 30261, Taiwan

J. Biomed. Opt. 21(8), 087003 (Aug 12, 2016). doi:10.1117/1.JBO.21.8.087003
History: Received May 10, 2016; Accepted July 27, 2016
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Abstract.  This study developed a microfluidic platform for replicating and detecting DNA in real time by integrating a laser and a microfluidic device composed of polydimethylsiloxane. The design of the microchannels consisted of a laser-heating area and a detection area. An infrared laser was used as the heating source for DNA replication, and the laser power was adjusted to heat the solutions directly. In addition, strong biotin–avidin binding was used to capture and detect the replicated products. The biotin on one end was bound to avidin and anchored to the surface of the microchannels, whereas the biotin on the other end was bound to the quantum dots (Qdots). The results showed that the fluorescent intensity of the Qdots bound to the replicated products in the detection area increased with the number of thermal cycles created by the laser. When the number of thermal cycles was 10, the fluorescent intensity of the Qdots was directly detectable on the surface of the microchannels. The proposed method is more sensitive than detection methods entailing gel electrophoresis.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Topics

Lasers

Citation

Min-Sheng Hung ; Chia-Chin Ho and Chih-Pin Chen
"Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection", J. Biomed. Opt. 21(8), 087003 (Aug 12, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.087003


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