Research Papers: Imaging

In vivo evaluation of adipose- and muscle-derived stem cells as a treatment for nonhealing diabetic wounds using multimodal microscopy

[+] Author Affiliations
Joanne Li, Marina Marjanovic

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States

University of Illinois at Urbana-Champaign, Department of Bioengineering, 1270 Digital Computer Laboratory MC-278, 1304 West Springfield Avenue, Urbana, Illinois 61801, United States

Yair Pincu, Marni D. Boppart

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States

University of Illinois at Urbana-Champaign, Department of Kinesiology and Community Health, 906 South Goodwin Avenue, Urbana, Illinois 61801, United States

Andrew J. Bower

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States

University of Illinois at Urbana-Champaign, Department of Electrical and Computer Engineering, 306 North Wright Street, Urbana, Illinois 61801, United States

Eric J. Chaney

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States

Tor Jensen

University of Illinois at Urbana-Champaign, Illinois Health Sciences Initiative, 611 West Park Street, Urbana, Illinois 61801, United States

Stephen A. Boppart

University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States

University of Illinois at Urbana-Champaign, Department of Bioengineering, 1270 Digital Computer Laboratory MC-278, 1304 West Springfield Avenue, Urbana, Illinois 61801, United States

University of Illinois at Urbana-Champaign, Department of Electrical and Computer Engineering, 306 North Wright Street, Urbana, Illinois 61801, United States

University of Illinois at Urbana-Champaign, Department of Internal Medicine, 506 South Mathews Avenue, Urbana, Illinois 61801, United States

J. Biomed. Opt. 21(8), 086006 (Aug 16, 2016). doi:10.1117/1.JBO.21.8.086006
History: Received April 14, 2016; Accepted July 28, 2016
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Abstract.  Impaired skin wound healing is a significant comorbid condition of diabetes, which often results in nonhealing diabetic ulcers due to poor peripheral microcirculation, among other factors. The effectiveness of the regeneration of adipose-derived stem cells (ADSCs) and muscle-derived stem cells (MDSCs) was assessed using an integrated multimodal microscopy system equipped with two-photon fluorescence and second-harmonic generation imaging. These imaging modalities, integrated in a single platform for spatial and temporal coregistration, allowed us to monitor in vivo changes in the collagen network and cell dynamics in a skin wound. Fluorescently labeled ADSCs and MDSCs were applied topically to the wound bed of wild-type and diabetic (db/db) mice following punch biopsy. Longitudinal imaging demonstrated that ADSCs and MDSCs provided remarkable capacity for improved diabetic wound healing, and integrated microscopy revealed a more organized collagen remodeling in the wound bed of treated mice. The results from this study verify the regenerative capacity of stem cells toward healing and, with multimodal microscopy, provide insight regarding their impact on the skin microenvironment. The optical method outlined in this study, which has the potential for in vivo human use, may optimize the care and treatment of diabetic nonhealing wounds.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Joanne Li ; Yair Pincu ; Marina Marjanovic ; Andrew J. Bower ; Eric J. Chaney, et al.
"In vivo evaluation of adipose- and muscle-derived stem cells as a treatment for nonhealing diabetic wounds using multimodal microscopy", J. Biomed. Opt. 21(8), 086006 (Aug 16, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.086006


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