Research Papers: Sensing

Aptamer-based fiber sensor for thrombin detection

[+] Author Affiliations
Luís Coelho, José Luís Santos, Pedro Alberto da Silva Jorge

University of Porto, CAP/INESC TEC—Technology and Science and FCUP—Faculty of Sciences, Rua do Campo Alegre, 4150-179 Porto, Portugal

José Manuel Marques Martins de Almeida

University of Porto, CAP/INESC TEC—Technology and Science and FCUP—Faculty of Sciences, Rua do Campo Alegre, 4150-179 Porto, Portugal

Universidade de Trás-os-Montes e Alto Douro, Department of Physics, School of Sciences and Technology, Apartado 1013, 5001-801 Vila Real, Portugal

Maria Cristina L. Martins

Universidade do Porto, i3S—Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto, INEB—Instituto de Engenharia Biomédica, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal

Universidade do Porto, ICBAS—Instituto de Ciências Biomédicas Abel Salazar, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal

Diana Viegas

Universidade do Porto, INEB—Instituto de Engenharia Biomédica, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal

Raquel B. Queirós

INL—International Iberian Nanotechnology, Avenida Mestre José Veiga s/n, 4715-330 Braga, Portugal

J. Biomed. Opt. 21(8), 087005 (Aug 18, 2016). doi:10.1117/1.JBO.21.8.087005
History: Received January 23, 2016; Accepted August 1, 2016
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Abstract.  The detection of thrombin based on aptamer binding is studied using two different optical fiber-based configurations: long period gratings coated with a thin layer of titanium dioxide and surface plasmon resonance devices in optical fibers coated with a multilayer of gold and titanium dioxide. These structures are functionalized and the performance to detect thrombin in the range 10 to 100 nM is compared in transmission mode. The sensitivity to the surrounding refractive index (RI) of the plasmonic device is higher than 3100  nmRIU1 in the RI range 1.335 to 1.355, a factor of 20 greater than the sensitivity of the coated grating. The detection of 10 nM of thrombin was accomplished with a wavelength shift of 3.5 nm and a resolution of 0.54 nM.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Luís Coelho ; José Manuel Marques Martins de Almeida ; José Luís Santos ; Pedro Alberto da Silva Jorge ; Maria Cristina L. Martins, et al.
"Aptamer-based fiber sensor for thrombin detection", J. Biomed. Opt. 21(8), 087005 (Aug 18, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.087005


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