Research Papers: Sensing

Table top surface plasmon resonance measurement system for efficient urea biosensing using ZnO thin film matrix

[+] Author Affiliations
Ayushi Paliwal, Vinay Gupta

University of Delhi, Department of Physics and Astrophysics, Delhi 110007, India

Monika Tomar

University of Delhi, Physics Department, Miranda House, Delhi 110007, India

J. Biomed. Opt. 21(8), 087006 (Aug 23, 2016). doi:10.1117/1.JBO.21.8.087006
History: Received May 11, 2016; Accepted August 2, 2016
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Abstract.  The present report addresses the application of surface plasmon resonance (SPR) phenomenon for urea sensing. The former promises a high sensitivity, label-free detection, and real-time information by monitoring the refractive index change at the metal–dielectric interface. In the present report, a highly sensitive urea biosensor has been developed by integrating a ZnO thin film matrix with the SPR technique. Kretschmann configuration has been used to excite the surface plasmon (SP) modes at the ZnO–metal (gold) interface using an indigeneously developed table top SPR measurement setup. Urease (Urs), the urea-specific enzyme, has been immobilized on the surface of ZnO thin film by physical adsorption technique. The SPR reflectance curves were recorded for the prism/Au/ZnO/Urs system in angular interrogation mode with phosphate-buffered saline (PBS) solution as the liquid media. The SPR resonance angle is found to be shifted toward a lower angle from 49.1 to 42.0 deg with an increase in the urea concentration (0 to 300  mg/dl) in the PBS solution. The developed sensor (prism/Au/ZnO/Urs) is found to be highly sensitive [sensitivity=0.039  deg/(mg/dl) or 203  deg/RIU] with detection accuracy of 0.045(deg)1.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Ayushi Paliwal ; Monika Tomar and Vinay Gupta
"Table top surface plasmon resonance measurement system for efficient urea biosensing using ZnO thin film matrix", J. Biomed. Opt. 21(8), 087006 (Aug 23, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.087006


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