Research Papers: Therapeutic

Macroscopic singlet oxygen modeling for dosimetry of Photofrin-mediated photodynamic therapy: an in-vivo study

[+] Author Affiliations
Haixia Qiu

Chinese PLA General Hospital, Department of Laser Medicine, No. 28 Fuxing Road, Haidian District, Beijing 100853, China

University of Pennsylvania, School of Medicine, Department of Radiation Oncology, 3400 Civic Center Boulevard TRC 4W, Philadelphia, Pennsylvania 19104, United States

Michele M. Kim

University of Pennsylvania, School of Medicine, Department of Radiation Oncology, 3400 Civic Center Boulevard TRC 4W, Philadelphia, Pennsylvania 19104, United States

University of Pennsylvania, Department of Physics and Astronomy, 209 South 33rd Street, Philadelphia, Pennsylvania 19104, United States

Rozhin Penjweini, Timothy C. Zhu

University of Pennsylvania, School of Medicine, Department of Radiation Oncology, 3400 Civic Center Boulevard TRC 4W, Philadelphia, Pennsylvania 19104, United States

J. Biomed. Opt. 21(8), 088002 (Aug 23, 2016). doi:10.1117/1.JBO.21.8.088002
History: Received April 15, 2016; Accepted August 1, 2016
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Abstract.  Although photodynamic therapy (PDT) is an established modality for cancer treatment, current dosimetric quantities, such as light fluence and PDT dose, do not account for the differences in PDT oxygen consumption for different fluence rates (φ). A macroscopic model was adopted to evaluate using calculated reacted singlet oxygen concentration ([O21]rx) to predict Photofrin-PDT outcome in mice bearing radiation-induced fibrosarcoma tumors, as singlet oxygen is the primary cytotoxic species responsible for cell death in type II PDT. Using a combination of fluences (50, 135, 200, and 250  J/cm2) and φ (50, 75, and 150  mW/cm2), tumor regrowth rate, k, was determined for each condition. A tumor cure index, CI=1k/kcontrol, was calculated based on the k between PDT-treated groups and that of the control, kcontrol. The measured Photofrin concentration and light dose for each mouse were used to calculate PDT dose and [O21]rx, while mean optical properties (μa=0.9  cm1, μs=8.4  cm1) were used to calculate φ for all mice. CI was correlated to the fluence, PDT dose, and [O21]rx with R2=0.35, 0.79, and 0.93, respectively. These results suggest that [O21]rx serves as a better dosimetric quantity for predicting PDT outcome.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Haixia Qiu ; Michele M. Kim ; Rozhin Penjweini and Timothy C. Zhu
"Macroscopic singlet oxygen modeling for dosimetry of Photofrin-mediated photodynamic therapy: an in-vivo study", J. Biomed. Opt. 21(8), 088002 (Aug 23, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.088002


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