Research Papers: Imaging

Calibration of optical coherence tomography angiography with a microfluidic chip

[+] Author Affiliations
Johnny P. Su, Rahul Chandwani, Simon S. Gao, Alex D. Pechauer, Miao Zhang, Jie Wang, Yali Jia, David Huang, Gangjun Liu

Oregon Health and Science University, Casey Eye Institute, 3375 Southwest Terwilliger Boulevard, Portland, Oregon 97239, United States

J. Biomed. Opt. 21(8), 086015 (Aug 24, 2016). doi:10.1117/1.JBO.21.8.086015
History: Received April 14, 2016; Accepted August 9, 2016
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Abstract.  A microfluidic chip with microchannels ranging from 8 to 96  μm was used to mimic blood vessels down to the capillary level. Blood flow within the microfluidic channels was analyzed with split-spectrum amplitude-decorrelation angiography (SSADA)-based optical coherence tomography (OCT) angiography. It was found that the SSADA decorrelation value was related to both blood flow speed and channel width. SSADA could differentiate nonflowing blood inside the microfluidic channels from static paper. The SSADA decorrelation value was approximately linear with blood flow velocity up to a threshold Vsat of 5.83±1.33  mm/s (mean±standard deviation over the range of channel widths). Beyond this threshold, it approached a saturation value Dsat. Dsat was higher for wider channels, and approached a maximum value Dsm as the channel width became much larger than the beam focal spot diameter. These results indicate that decorrelation values (flow signal) in capillary networks would be proportional to both flow velocity and vessel caliber but would be capped at a saturation value in larger blood vessels. These findings are useful for interpretation and quantification of clinical OCT angiography results.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Johnny P. Su ; Rahul Chandwani ; Simon S. Gao ; Alex D. Pechauer ; Miao Zhang, et al.
"Calibration of optical coherence tomography angiography with a microfluidic chip", J. Biomed. Opt. 21(8), 086015 (Aug 24, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.086015


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