Research Papers: Imaging

Quantitative index imaging of coculture cells by scanning focused refractive index microscopy

[+] Author Affiliations
Teng-Qian Sun, Fen Hu, Shi-ke Liu, Xiao-Wan Wang, Jin Wang, Zhi-Chao Deng, Chun-Ping Zhang, Xin-Yu Wang

Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China

Qing Ye, Wen-Yuan Zhou, Jian-Guo Tian

Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China

Nankai University, The 2011 Project Collaborative Innovation Center for Biological Therapy, 94th Weijin Road, Tianjin 300071, China

Jian-Chun Mei

Nankai University, The 2011 Project Collaborative Innovation Center for Biological Therapy, 94th Weijin Road, Tianjin 300071, China

Nankai University, Advanced Technology Institute, 94th Weijin Road, Tianjin 300071, China

Lei-Ting Pan

Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China

Nankai University, State Key Laboratory of Medicinal Chemical Biology, 94th Weijin Road, Tianjin, China

J. Biomed. Opt. 21(8), 086016 (Aug 26, 2016). doi:10.1117/1.JBO.21.8.086016
History: Received April 13, 2016; Accepted August 15, 2016
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Abstract.  We report the quantitative refractive index (RI) imaging of cocultured cells in their living environment by scanning focused refractive index microscopy (SFRIM). Mouse microglial cells and synovial cells are cocultured on the top surface of a trapezoid prism. The RI imaging of living cells is obtained in a reflection-type method. The RI information is deduced with the simple derivative total internal reflection method, where a complex retrieval algorithm or reconstruction process is unnecessary. The outline of each cell is determined according to the RI value compared with that of the immersion liquid. The cocultured cells can be discriminated in the RI image. The measurement is nondestructive and label-free. The experimental results prove that SFRIM is a promising tool in the field of biological optics.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Teng-Qian Sun ; Qing Ye ; Fen Hu ; Shi-ke Liu ; Xiao-Wan Wang, et al.
"Quantitative index imaging of coculture cells by scanning focused refractive index microscopy", J. Biomed. Opt. 21(8), 086016 (Aug 26, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.8.086016


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