JBO Letters

Integrated quantitative phase and birefringence microscopy for imaging malaria-infected red blood cells

[+] Author Affiliations
Chengshuai Li, Shichao Chen, Yizheng Zhu

Virginia Tech, The Bradley Department of Electrical and Computer Engineering, 1185 Perry Street, Blacksburg, Virginia 24061, United States

Michael Klemba

Virginia Tech, Department of Biochemistry, 340 West Campus Drive, Blacksburg, Virginia 24061, United States

J. Biomed. Opt. 21(9), 090501 (Sep 06, 2016). doi:10.1117/1.JBO.21.9.090501
History: Received April 21, 2016; Accepted August 15, 2016
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Abstract

Abstract.  A dual-modality birefringence/phase imaging system is presented. The system features a crystal retarder that provides polarization mixing and generates two interferometric carrier waves in a single signal spectrum. The retardation and orientation of sample birefringence can then be measured simultaneously based on spectral multiplexing interferometry. Further, with the addition of a Nomarski prism, the same setup can be used for quantitative differential interference contrast (DIC) imaging. Sample phase can then be obtained with two-dimensional integration. In addition, birefringence-induced phase error can be corrected using the birefringence data. This dual-modality approach is analyzed theoretically with Jones calculus and validated experimentally with malaria-infected red blood cells. The system generates not only corrected DIC and phase images, but a birefringence map that highlights the distribution of hemozoin crystals.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Chengshuai Li ; Shichao Chen ; Michael Klemba and Yizheng Zhu
"Integrated quantitative phase and birefringence microscopy for imaging malaria-infected red blood cells", J. Biomed. Opt. 21(9), 090501 (Sep 06, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.9.090501


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