Research Papers: Imaging

Upconversion microparticles as time-resolved luminescent probes for multiphoton microscopy: desired signal extraction from the streaking effect

[+] Author Affiliations
Daria V. Pominova, Pavel V. Grachev, Igor D. Romanishkin, Sergei V. Kuznetsov, Julia A. Rozhnova, Daria S. Yasyrkina, Pavel P. Fedorov

A. M. Prokhorov General Physics Institute, Russian Academy of Sciences, Vavilov Street, 38, Moscow 119991, Russia

Anastasia V. Ryabova, Victor B. Loschenov

A. M. Prokhorov General Physics Institute, Russian Academy of Sciences, Vavilov Street, 38, Moscow 119991, Russia

National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Kashirskoe Highway, 31, Moscow, 115409, Russia

J. Biomed. Opt. 21(9), 096002 (Sep 08, 2016). doi:10.1117/1.JBO.21.9.096002
History: Received June 2, 2016; Accepted August 10, 2016
Text Size: A A A

Abstract.  The great interest in upconversion nanoparticles exists due to their high efficiency under multiphoton excitation. However, when these particles are used in scanning microscopy, the upconversion luminescence causes a streaking effect due to the long lifetime. This article describes a method of upconversion microparticle luminescence lifetime determination with help of modified Lucy–Richardson deconvolution of laser scanning microscope (LSM) image obtained under near-IR excitation using nondescanned detectors. Determination of the upconversion luminescence intensity and the decay time of separate microparticles was done by intensity profile along the image fast scan axis approximation. We studied upconversion submicroparticles based on fluoride hosts doped with Yb3+-Er3+ and Yb3+-Tm3+ rare earth ion pairs, and the characteristic decay times were 0.1 to 1.5 ms. We also compared the results of LSM measurements with the photon counting method results; the spread of values was about 13% and was associated with the approximation error. Data obtained from live cells showed the possibility of distinguishing the position of upconversion submicroparticles inside and outside the cells by the difference of their lifetime. The proposed technique allows using the upconversion microparticles without shells as probes for the presence of OH ions and CO2 molecules.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Daria V. Pominova ; Anastasia V. Ryabova ; Pavel V. Grachev ; Igor D. Romanishkin ; Sergei V. Kuznetsov, et al.
"Upconversion microparticles as time-resolved luminescent probes for multiphoton microscopy: desired signal extraction from the streaking effect", J. Biomed. Opt. 21(9), 096002 (Sep 08, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.9.096002


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.