Research Papers: Imaging

Scattering correction through a space-variant blind deconvolution algorithm

[+] Author Affiliations
Koberstein-Schwarz Benno

Carl Zeiss AG, Corporate Research and Technology, Carl-Zeiss-Promenade 10, 07745 Jena, Germany

Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum, München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany

Omlor Lars, Schmitt-Manderbach Tobias

Carl Zeiss AG, Corporate Research and Technology, Carl-Zeiss-Promenade 10, 07745 Jena, Germany

Mappes Timo

Carl Zeiss Vision International GmbH, Technology and Innovation, Turnstr. 27, 73430 Aalen, Germany

Ntziachristos Vasilis

Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum, München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany

J. Biomed. Opt. 21(9), 096005 (Sep 12, 2016). doi:10.1117/1.JBO.21.9.096005
History: Received April 28, 2016; Accepted August 15, 2016
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Abstract.  Scattering within biological samples limits the imaging depth and the resolution in microscopy. We present a prior and regularization approach for blind deconvolution algorithms to correct the influence of scattering to increase the imaging depth and resolution. The effect of the prior is demonstrated on a three-dimensional image stack of a zebrafish embryo captured with a selective plane illumination microscope. Blind deconvolution algorithms model the recorded image as a convolution between the distribution of fluorophores and a point spread function (PSF). Our prior uses image information from adjacent z-planes to estimate the unknown blur in tissue. The increased size of the PSF due to the cascading effect of scattering in deeper tissue is accounted for by a depth adaptive regularizer model. In a zebrafish sample, we were able to extend the point in depth, where scattering has a significant effect on the image quality by around 30  μm.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Koberstein-Schwarz Benno ; Omlor Lars ; Schmitt-Manderbach Tobias ; Mappes Timo and Ntziachristos Vasilis
"Scattering correction through a space-variant blind deconvolution algorithm", J. Biomed. Opt. 21(9), 096005 (Sep 12, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.9.096005


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