Research Papers: Imaging

In vivo optical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

[+] Author Affiliations
Benquan Wang, Yiming Lu

University of Illinois at Chicago, Department of Bioengineering, 851 South Morgan Street, Chicago, Illinois 60607, United States

Xincheng Yao

University of Illinois at Chicago, Department of Bioengineering, 851 South Morgan Street, Chicago, Illinois 60607, United States

University of Illinois at Chicago, Department of Ophthalmology and Visual Sciences, 1855 West Taylor Street, Chicago, Illinois 60612, United States

J. Biomed. Opt. 21(9), 096010 (Sep 21, 2016). doi:10.1117/1.JBO.21.9.096010
History: Received June 16, 2016; Accepted August 26, 2016
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Abstract.  Intrinsic optical signal (IOS) imaging promises a noninvasive method for advanced study and diagnosis of eye diseases. Before pursuing clinical applications, it is essential to understand anatomic and physiological sources of retinal IOSs and to establish the relationship between IOS distortions and eye diseases. The purpose of this study was designed to demonstrate the feasibility of in vivo IOS imaging of mouse models. A high spatiotemporal resolution spectral domain optical coherence tomography (SD-OCT) was employed for depth-resolved retinal imaging. A custom-designed animal holder equipped with ear bar and bite bar was used to minimize eye movements. Dynamic OCT imaging revealed rapid IOS from the photoreceptor’s outer segment immediately after the stimulation delivery, and slow IOS changes were observed from inner retinal layers. Comparative photoreceptor IOS and electroretinography recordings suggested that the fast photoreceptor IOS may be attributed to the early stage of phototransduction before the hyperpolarization of retinal photoreceptor.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Benquan Wang ; Yiming Lu and Xincheng Yao
"In vivo optical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas", J. Biomed. Opt. 21(9), 096010 (Sep 21, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.9.096010


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