JBO Letters

Profiling of individual human red blood cells under osmotic stress using defocusing microscopy

[+] Author Affiliations
Paula M. S. Roma, Livia Siman, Ubirajara Agero, Oscar N. Mesquita

Universidade Federal de Minas Gerais, Departamento de Física, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil

Barbara Hissa

University of Chicago, James Franck Institute, Institute for Biophysical Dynamics and Physics Department, Chicago, Illinois 60637, United States

Erika M. Braga

Universidade Federal de Minas Gerais, Departamento de Parasitologia, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil

J. Biomed. Opt. 21(9), 090505 (Sep 21, 2016). doi:10.1117/1.JBO.21.9.090505
History: Received June 17, 2016; Accepted August 15, 2016
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Abstract.  We use a quantitative phase imaging technique, defocusing microscopy (DM), to measure morphological, chemical, and mechanical parameters of individual red blood cells (RBCs) immersed in solutions with different osmolalities. We monitor the RBCs’ radius, volume, surface area, sphericity index, and hemoglobin content and concentration. The complete shape of cells is recovered and the effects of their adhesion to the glass substrate are observed. Finally, membrane fluctuation measurements give us information about the cells deformability.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Paula M. S. Roma ; Livia Siman ; Barbara Hissa ; Ubirajara Agero ; Erika M. Braga, et al.
"Profiling of individual human red blood cells under osmotic stress using defocusing microscopy", J. Biomed. Opt. 21(9), 090505 (Sep 21, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.9.090505


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