Research Papers: General

Mueller matrix approach for probing multifractality in the underlying anisotropic connective tissue

[+] Author Affiliations
Nandan Kumar Das, Rajib Dey, Nirmalya Ghosh

Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur 741246, West Bengal, India

J. Biomed. Opt. 21(9), 095004 (Sep 26, 2016). doi:10.1117/1.JBO.21.9.095004
History: Received June 3, 2016; Accepted August 29, 2016
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Abstract.  Spatial variation of refractive index (RI) in connective tissues exhibits multifractality, which encodes useful morphological and ultrastructural information about the disease. We present a spectral Mueller matrix (MM)-based approach in combination with multifractal detrended fluctuation analysis (MFDFA) to exclusively pick out the signature of the underlying connective tissue multifractality through the superficial epithelium layer. The method is based on inverse analysis on selected spectral scattering MM elements encoding the birefringence information on the anisotropic connective tissue. The light scattering spectra corresponding to the birefringence carrying MM elements are then subjected to the Born approximation-based Fourier domain preprocessing to extract ultrastructural RI fluctuations of anisotropic tissue. The extracted RI fluctuations are subsequently analyzed via MFDFA to yield the multifractal tissue parameters. The approach was experimentally validated on a simple tissue model comprising of TiO2 as scatterers of the superficial isotropic layer and rat tail collagen as an underlying anisotropic layer. Finally, the method enabled probing of precancer-related subtle alterations in underlying connective tissue ultrastructural multifractality from intact tissues.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Nandan Kumar Das ; Rajib Dey and Nirmalya Ghosh
"Mueller matrix approach for probing multifractality in the underlying anisotropic connective tissue", J. Biomed. Opt. 21(9), 095004 (Sep 26, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.9.095004


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