Special Series on Translational Biophotonics

Super-resolution optical microscopy study of telomere structure

[+] Author Affiliations
Mary Lisa Phipps, Peter M. Goodwin

Los Alamos National Laboratory, Center for Integrated Nanotechnologies, P.O. Box 1663, Bikini Atoll Road, SM-30, Los Alamos, New Mexico 84545, United States

Jennifer S. Martinez

Los Alamos National Laboratory, Center for Integrated Nanotechnologies, P.O. Box 1663, Bikini Atoll Road, SM-30, Los Alamos, New Mexico 84545, United States

Los Alamos National Laboratory, Institute for Materials Science, P.O. Box 1663, Bikini Atoll Road, SM-30, Los Alamos, New Mexico 84545, United States

Edwin H. Goodwin

The New Mexico Consortium, 100 Entrada Drive, Los Alamos, New Mexico 87544, United States

J. Biomed. Opt. 21(9), 094003 (Sep 29, 2016). doi:10.1117/1.JBO.21.9.094003
History: Received June 17, 2016; Accepted September 7, 2016
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Abstract.  Chromosome ends are shielded from exonucleolytic attack and inappropriate end-joining by terminal structures called telomeres; these structures are potential targets for anticancer drugs. Telomeres are composed of a simple DNA sequence (5′-TTAGGG-3′ in humans) repeated more than a thousand times, a short 3′ single-stranded overhang, and numerous proteins. Electron microscopy has shown that the 3′ overhang pairs with the complementary strand at an internal site creating a small displacement loop and a large double-stranded “t-loop.” Our goal is to determine whether all telomeres adopt the t-loop configuration, or whether there are two or more distinct configurations. Progress in optimizing super-resolution (SR) microscopy for this ongoing investigation is reported here. Results suggest that under certain conditions sample preparation procedures may disrupt chromatin by causing loss of nucleosomes. This finding may limit the use of SR microscopy in telomere studies.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Mary Lisa Phipps ; Peter M. Goodwin ; Jennifer S. Martinez and Edwin H. Goodwin
"Super-resolution optical microscopy study of telomere structure", J. Biomed. Opt. 21(9), 094003 (Sep 29, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.9.094003


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