Research Papers: General

Multiplexed fluorescence mediated tomography with temporal and spectral data

[+] Author Affiliations
Ying Mu, Vivian Pera, Mark Niedre

Northeastern University, Department of Electrical and Computer Engineering, 360 Huntington Avenue, Boston, Massachusetts 02115, United States

J. Biomed. Opt. 21(10), 105001 (Oct 04, 2016). doi:10.1117/1.JBO.21.10.105001
History: Received July 6, 2016; Accepted August 31, 2016
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Abstract.  We recently developed an algorithm for multiplexed fluorescence tomographic imaging of at least four fluorophores concurrently in the red and near-infrared wavelength region by jointly using spectral and temporal data. We report the design of a fluorescence tomography instrument that acquires spectral and temporal data, and validate its use in tissue-mimicking phantoms with four embedded fluorescent targets with highly overlapped spectral signatures. Critically, this requires measurement or computation of extended fluorophore signature libraries, which capture the variability in the measured signal due to the unknown position of the targets in the media. We demonstrate that we can demix and tomographically image all four fluorophores with zero image cross-talk, and 1 mm or better spatial resolution.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Ying Mu ; Vivian Pera and Mark Niedre
"Multiplexed fluorescence mediated tomography with temporal and spectral data", J. Biomed. Opt. 21(10), 105001 (Oct 04, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.10.105001


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