Special Section on Adaptive Optics and Wavefront Control for Biological Systems

Investigation on dynamics of red blood cells through their behavior as biophotonic lenses

[+] Author Affiliations
Pasquale Memmolo, Francesco Merola, Lisa Miccio, Martina Mugnano, Pietro Ferraro

National Council of Research-Istituto di Scienze Applicate e Sistemi Intelligenti “E. Caianiello,” Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy

J. Biomed. Opt. 21(12), 121509 (Oct 13, 2016). doi:10.1117/1.JBO.21.12.121509
History: Received June 13, 2016; Accepted September 19, 2016
Text Size: A A A

Abstract.  The possibility to adopt biological matter as photonic optical elements can open scenarios in biophotonics research. Recently, it has been demonstrated that a red blood cell (RBC) can be seen as an optofluidic microlens by showing its imaging capability as well as its focal tunability. Moreover, correlation between an RBC’s morphology and its behavior as a refractive optical element has been established and its exploitation for biomedical diagnostic purposes has been foreseen. In fact, any deviation from the healthy RBC morphology can be seen as additional aberration in the optical wavefront passing through the cell. By this concept, accurate localization of focal spots of RBCs can become very useful in the blood disorders identification. We investigate the three-dimensional positioning of such focal spots over time for samples with two different osmolarity conditions, i.e., when they assume discocyte and spherical shapes, respectively. We also demonstrate that a temporal variation of an RBC’s focal points along the optical axis is correlated to the temporal fluctuations in the RBC’s thickness maps. Furthermore, we show a sort of synchronization of the whole erythrocytes ensemble.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Topics

Blood ; Lenses

Citation

Pasquale Memmolo ; Francesco Merola ; Lisa Miccio ; Martina Mugnano and Pietro Ferraro
"Investigation on dynamics of red blood cells through their behavior as biophotonic lenses", J. Biomed. Opt. 21(12), 121509 (Oct 13, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.12.121509


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.