Research Papers: Sensing

Reflected scatterometry for noninvasive interrogation of bacterial colonies

[+] Author Affiliations
Huisung Kim, Iyll-Joon Doh, Euiwon Bae

Purdue University, School of Mechanical Engineering, Applied Optics Laboratory, West Lafayette, Indiana 47907, United States

Jennifer Sturgis

Purdue University, Department of Basic Medical Sciences, West Lafayette, Indiana 47907, United States

Arun K. Bhunia

Purdue University, Molecular Food Microbiology Laboratory, Department of Food Science, West Lafayette, Indiana 47907, United States

J. Paul Robinson

Purdue University, Department of Basic Medical Sciences, West Lafayette, Indiana 47907, United States

Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, United States

J. Biomed. Opt. 21(10), 107004 (Oct 24, 2016). doi:10.1117/1.JBO.21.10.107004
History: Received April 8, 2016; Accepted October 6, 2016
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Abstract.  A phenotyping of bacterial colonies on agar plates using forward-scattering diffraction-pattern analysis provided promising classification of several different bacteria such as Salmonella, Vibrio, Listeria, and E. coli. Since the technique is based on forward-scattering phenomena, light transmittance of both the colony and the medium is critical to ensure quality data. However, numerous microorganisms and their growth media allow only limited light penetration and render the forward-scattering measurement a challenging task. For example, yeast, Lactobacillus, mold, and several soil bacteria form colorful and dense colonies that obstruct most of the incoming light passing through them. Moreover, blood agar, which is widely utilized in the clinical field, completely blocks the incident coherent light source used in forward scatterometry. We present a newly designed reflection scatterometer and validation of the resolving power of the instrument. The reflectance-type instrument can acquire backward elastic scatter patterns for both highly opaque media and colonies and has been tested with three different bacterial genera grown on blood agar plates. Cross-validation results show a classification rate above 90% for four genera.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Huisung Kim ; Iyll-Joon Doh ; Jennifer Sturgis ; Arun K. Bhunia ; J. Paul Robinson, et al.
"Reflected scatterometry for noninvasive interrogation of bacterial colonies", J. Biomed. Opt. 21(10), 107004 (Oct 24, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.10.107004


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