Research Papers: General

Photodynamic therapy-induced nitric oxide production in neuronal and glial cells

[+] Author Affiliations
Vera D. Kovaleva, Anatoly B. Uzdensky

Southern Federal University, Laboratory of Molecular Neurobiology, Academy of Biology and Biotechnology, Stachky Avenue 194/1, 344090 Rostov-on-Don, Russia

J. Biomed. Opt. 21(10), 105005 (Oct 26, 2016). doi:10.1117/1.JBO.21.10.105005
History: Received June 6, 2016; Accepted October 5, 2016
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Abstract.  Nitric oxide (NO) has been recently demonstrated to enhance apoptosis of glial cells induced by photodynamic therapy (PDT), but to protect glial cells from PDT-induced necrosis in the crayfish stretch receptor, a simple neuroglial preparation that consists of a single mechanosensory neuron enveloped by satellite glial cells. We used the NO-sensitive fluorescent probe 4,5-diaminofluorescein diacetate to study the distribution and dynamics of PDT-induced NO production in the mechanosensory neuron and surrounding glial cells. The NO production in the glial envelope was higher than in the neuronal soma axon and dendrites both in control and in experimental conditions. In dark NO generator, DEA NONOate or NO synthase substrate L-arginine hydrochloride significantly increased the NO level in glial cells, whereas NO scavenger 2-Phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO) or inhibitors of NO synthase L-NG-nitro arginine methyl ester and Nω-nitro-L-arginine decreased it. PDT induced the transient increase in NO production with a maximum at 4 to 7 min after the irradiation start followed by its inhibition at 10 to 40 min. We suggested that PDT stimulated neuronal rather than inducible NO synthase isoform in glial cells, and the produced NO could mediate PDT-induced apoptosis.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Vera D. Kovaleva and Anatoly B. Uzdensky
"Photodynamic therapy-induced nitric oxide production in neuronal and glial cells", J. Biomed. Opt. 21(10), 105005 (Oct 26, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.10.105005


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