A laparoscopic MSI system developed by our group, and characterized and validated in a separate publication,6 has been used in the previous pilot studies of uterine transplantation procedures.13,14 The clinical imaging system comprises a 30-deg laparoscope (Karl Storz GmbH, Tuttlingen, Germany), LCTF (Varispec, Cri, Inc.), and monochrome camera (DCU223M, Thorlabs Ltd., UK). A surgical xenon light source (xenon 300, Karl Storz GmbH, Tuttlingen, Germany) was used for illumination. The laparoscope was secured to a flexible mounting arm and the whole system placed on a trolley that could be conveniently positioned at the bedside for measurements. A data cube of 13 images (500 to 620 nm) was acquired to generate reflected intensity spectra of the tissue at each spatial location. Exposure times of were required, resulting in a total acquisition time of . A preprocessing image registration step using custom-written feature-tracking software15,16 was carried out to compensate for misalignments caused by breathing and peristalsis motion during this time. The spectral sensitivity of the optical system was corrected for using a white reflectance target (Spectralon, Labsphere, Inc.) before converting the data to absorbance spectra. Figure 1 shows the laparoscope in position during imaging of the rabbit uterus.