Research Papers: Imaging

Spatially multiplexed interferometric microscopy with partially coherent illumination

[+] Author Affiliations
José Ángel Picazo-Bueno, Javier García, Carlos Ferreira, Vicente Micó

Universitat de Valencia, Departamento de Óptica, C/Doctor Moliner 50, Burjassot 46100, Spain

Zeev Zalevsky

Bar-Ilan University, Faculty of Engineering, Ramat-Gan 52900, Israel

J. Biomed. Opt. 21(10), 106007 (Oct 27, 2016). doi:10.1117/1.JBO.21.10.106007
History: Received May 3, 2016; Accepted October 6, 2016
Text Size: A A A

Abstract.  We have recently reported on a simple, low cost, and highly stable way to convert a standard microscope into a holographic one [Opt. Express 22, 14929 (2014)]. The method, named spatially multiplexed interferometric microscopy (SMIM), proposes an off-axis holographic architecture implemented onto a regular (nonholographic) microscope with minimum modifications: the use of coherent illumination and a properly placed and selected one-dimensional diffraction grating. In this contribution, we report on the implementation of partially (temporally reduced) coherent illumination in SMIM as a way to improve quantitative phase imaging. The use of low coherence sources forces the application of phase shifting algorithm instead of off-axis holographic recording to recover the sample’s phase information but improves phase reconstruction due to coherence noise reduction. In addition, a less restrictive field of view limitation (1/2) is implemented in comparison with our previously reported scheme (1/3). The proposed modification is experimentally validated in a regular Olympus BX-60 upright microscope considering a wide range of samples (resolution test, microbeads, swine sperm cells, red blood cells, and prostate cancer cells).

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

José Ángel Picazo-Bueno ; Zeev Zalevsky ; Javier García ; Carlos Ferreira and Vicente Micó
"Spatially multiplexed interferometric microscopy with partially coherent illumination", J. Biomed. Opt. 21(10), 106007 (Oct 27, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.10.106007


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.