Special Series on Translational Biophotonics

Hyperspectral imaging fluorescence excitation scanning for colon cancer detection

[+] Author Affiliations
Silas J. Leavesley

University of South Alabama, Department of Chemical and Biomolecular Engineering, 150 Jaguar Drive, SH 4129, Mobile, Alabama 36688, United States

University of South Alabama, Department of Pharmacology, 5851 USA North Drive, MSB 3372, Mobile, Alabama 36688, United States

University of South Alabama, Center for Lung Biology, 5851 USA North Drive, MSB 3340, Mobile, Alabama 36688, United States

Mikayla Walters

University of South Alabama, Department of Chemical and Biomolecular Engineering, 150 Jaguar Drive, SH 4129, Mobile, Alabama 36688, United States

Carmen Lopez

University of South Alabama, Medical Sciences Program, 5851 USA North Drive, MSB 3340, Mobile, Alabama 36688, United States

Thomas Baker

University of South Alabama, Department of Pharmacology, 5851 USA North Drive, MSB 3372, Mobile, Alabama 36688, United States

Peter F. Favreau

University of South Alabama, Department of Chemical and Biomolecular Engineering, 150 Jaguar Drive, SH 4129, Mobile, Alabama 36688, United States

University of South Alabama, Center for Lung Biology, 5851 USA North Drive, MSB 3340, Mobile, Alabama 36688, United States

Thomas C. Rich

University of South Alabama, Department of Pharmacology, 5851 USA North Drive, MSB 3372, Mobile, Alabama 36688, United States

University of South Alabama, Center for Lung Biology, 5851 USA North Drive, MSB 3340, Mobile, Alabama 36688, United States

Paul F. Rider

University of South Alabama, Department of Surgery, 2451 Fillingim Street, Mastin Building, Suite 701, Mobile, Alabama 36617, United States

Carole W. Boudreaux

University of South Alabama, Department of Pathology, 2451 Fillingim Street, Mobile, Alabama 36617, United States

J. Biomed. Opt. 21(10), 104003 (Oct 28, 2016). doi:10.1117/1.JBO.21.10.104003
History: Received May 25, 2016; Accepted October 4, 2016
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Abstract.  Optical spectroscopy and hyperspectral imaging have shown the potential to discriminate between cancerous and noncancerous tissue with high sensitivity and specificity. However, to date, these techniques have not been effectively translated to real-time endoscope platforms. Hyperspectral imaging of the fluorescence excitation spectrum represents new technology that may be well suited for endoscopic implementation. However, the feasibility of detecting differences between normal and cancerous mucosa using fluorescence excitation-scanning hyperspectral imaging has not been evaluated. The goal of this study was to evaluate the initial feasibility of using fluorescence excitation-scanning hyperspectral imaging for measuring changes in fluorescence excitation spectrum concurrent with colonic adenocarcinoma using a small pre-pilot-scale sample size. Ex vivo analysis was performed using resected pairs of colorectal adenocarcinoma and normal mucosa. Adenocarcinoma was confirmed by histologic evaluation of hematoxylin and eosin (H&E) permanent sections. Specimens were imaged using a custom hyperspectral imaging fluorescence excitation-scanning microscope system. Results demonstrated consistent spectral differences between normal and cancerous tissues over the fluorescence excitation range of 390 to 450 nm that could be the basis for wavelength-dependent detection of colorectal cancers. Hence, excitation-scanning hyperspectral imaging may offer an alternative approach for discriminating adenocarcinoma from surrounding normal colonic mucosa, but further studies will be required to evaluate the accuracy of this approach using a larger patient cohort.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Silas J. Leavesley ; Mikayla Walters ; Carmen Lopez ; Thomas Baker ; Peter F. Favreau, et al.
"Hyperspectral imaging fluorescence excitation scanning for colon cancer detection", J. Biomed. Opt. 21(10), 104003 (Oct 28, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.10.104003


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