Research Papers: Sensing

Rapid diagnosis and differentiation of microbial pathogens in otitis media with a combined Raman spectroscopy and low-coherence interferometry probe: toward in vivo implementation

[+] Author Affiliations
Youbo Zhao, Ryan L. Shelton, Ryan M. Nolan, Haohua Tu, Eric J. Chaney

Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States

Guillermo L. Monroy, Sixian You

Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States

University of Illinois at Urbana–Champaign, Department of Bioengineering, 1304 West Springfield Avenue, Urbana, Illinois 61801, United States

Stephen A. Boppart

Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States

University of Illinois at Urbana–Champaign, Department of Bioengineering, 1304 West Springfield Avenue, Urbana, Illinois 61801, United States

University of Illinois at Urbana–Champaign, Department of Electrical and Computer Engineering, 306 North Wright Street, Urbana, Illinois 61801, United States

University of Illinois at Urbana–Champaign, Department of Internal Medicine, 506 South Mathews Avenue, Urbana, Illinois 61801, United States

J. Biomed. Opt. 21(10), 107005 (Oct 31, 2016). doi:10.1117/1.JBO.21.10.107005
History: Received July 20, 2016; Accepted October 7, 2016
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Abstract.  We investigate and demonstrate the feasibility of using a combined Raman scattering (RS) spectroscopy and low-coherence interferometry (LCI) probe to differentiate microbial pathogens and improve our diagnostic ability of ear infections [otitis media (OM)]. While the RS probe provides noninvasive molecular information to identify and differentiate infectious microorganisms, the LCI probe helps to identify depth-resolved structural information as well as to guide and monitor positioning of the Raman spectroscopy beam for relatively longer signal acquisition times. A series of phantom studies, including the use of human middle ear effusion samples, were performed to mimic the conditions of in vivo investigations. These were also conducted to validate the feasibility of using this combined RS/LCI probe for point-of-care diagnosis of the infectious pathogen(s) in OM patients. This work establishes important parameters for future in vivo investigations of fast and accurate determination and diagnosis of infectious microorganisms in OM patients, potentially improving the efficacy and outcome of OM treatments, and importantly reducing the misuse of antibiotics in the presence of viral infections.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Youbo Zhao ; Guillermo L. Monroy ; Sixian You ; Ryan L. Shelton ; Ryan M. Nolan, et al.
"Rapid diagnosis and differentiation of microbial pathogens in otitis media with a combined Raman spectroscopy and low-coherence interferometry probe: toward in vivo implementation", J. Biomed. Opt. 21(10), 107005 (Oct 31, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.10.107005


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