Research Papers: Imaging

Changes in dynamic embryonic heart wall motion in response to outflow tract banding measured using video densitometry

[+] Author Affiliations
Stephanie Stovall, Madeline Midgett

Oregon Health and Science University, Department of Biomedical Engineering, 3303 SW Bond Avenue, Mail Code CH13B, Portland, Oregon 97239, United States

Kent Thornburg

Oregon Health and Science University, Center for Developmental Health, Knight Cardiovascular Institute, 3303 SW Bond Avenue, Mail Code CH13B, Portland, Oregon 97239, United States

Sandra Rugonyi

Oregon Health and Science University, Department of Biomedical Engineering, 3303 SW Bond Avenue, Mail Code CH13B, Portland, Oregon 97239, United States

Oregon Health and Science University, Center for Developmental Health, Knight Cardiovascular Institute, 3303 SW Bond Avenue, Mail Code CH13B, Portland, Oregon 97239, United States

J. Biomed. Opt. 21(11), 116003 (Nov 04, 2016). doi:10.1117/1.JBO.21.11.116003
History: Received June 28, 2016; Accepted October 18, 2016
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Abstract.  Abnormal blood flow during early cardiovascular development has been identified as a key factor in the pathogenesis of congenital heart disease; however, the mechanisms by which altered hemodynamics induce cardiac malformations are poorly understood. This study used outflow tract (OFT) banding to model increased afterload, pressure, and blood flow velocities at tubular stages of heart development and characterized the immediate changes in cardiac wall motion due to banding in chicken embryo models with light microscopy-based video densitometry. Optical videos were used to acquire two-dimensional heart image sequences over the cardiac cycle, from which intensity data were extracted along the heart centerline at several locations in the heart ventricle and OFT. While no changes were observed in the synchronous contraction of the ventricle with banding, the peristaltic-like wall motion in the OFT was significantly affected. Our data provide valuable insight into early cardiac biomechanics and its characterization using a simple light microscopy-based imaging modality.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Topics

Heart ; Video

Citation

Stephanie Stovall ; Madeline Midgett ; Kent Thornburg and Sandra Rugonyi
"Changes in dynamic embryonic heart wall motion in response to outflow tract banding measured using video densitometry", J. Biomed. Opt. 21(11), 116003 (Nov 04, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.11.116003


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