Research Papers: Imaging

Nondestructive imaging of fiber structure in articular cartilage using optical polarization tractography

[+] Author Affiliations
Xuan Yao, Yuanbo Wang, Mohammadreza Ravanfar, Gang Yao

University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United States

Ferris M. Pfeiffer

University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United States

University of Missouri, Department of Orthopedic Surgery, 1100 Virginia Avenue, Columbia 65212, United States

Dongsheng Duan

University of Missouri, Department of Bioengineering, 1406 E. Rollins Street, Columbia 65211, United States

University of Missouri, Department of Molecular Microbiology and Immunology, One Hospital Drive, Columbia 65212, United States

J. Biomed. Opt. 21(11), 116004 (Nov 07, 2016). doi:10.1117/1.JBO.21.11.116004
History: Received September 1, 2016; Accepted October 17, 2016
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Abstract.  Collagen fiber orientation plays an important role in determining the structure and function of the articular cartilage. However, there is currently a lack of nondestructive means to image the fiber orientation from the cartilage surface. The purpose of this study is to investigate whether the newly developed optical polarization tractography (OPT) can image fiber structure in articular cartilage. OPT was applied to obtain the depth-dependent fiber orientation in fresh articular cartilage samples obtained from porcine phalanges. For comparison, we also obtained collagen fiber orientation in the superficial zone of the cartilage using the established split-line method. The direction of each split-line was quantified using image processing. The orientation measured in OPT agreed well with those obtained from the split-line method. The correlation analysis of a total of 112 split-lines showed a greater than 0.9 coefficient of determination (R2) between the split-line results and OPT measurements obtained between 40 and 108  μm in depth. In addition, the thickness of the superficial layer can also be assessed from the birefringence images obtained in OPT. These results support that OPT provides a nondestructive way to image the collagen fiber structure in articular cartilage. This technology may be valuable for both basic cartilage research and clinical orthopedic applications.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Xuan Yao ; Yuanbo Wang ; Mohammadreza Ravanfar ; Ferris M. Pfeiffer ; Dongsheng Duan, et al.
"Nondestructive imaging of fiber structure in articular cartilage using optical polarization tractography", J. Biomed. Opt. 21(11), 116004 (Nov 07, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.11.116004


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