Research Papers: Imaging

Noninvasive in vivo multispectral optoacoustic imaging of apoptosis in triple negative breast cancer using indocyanine green conjugated phosphatidylserine monoclonal antibody

[+] Author Affiliations
Ravi Kumar Kannadorai, Yu Wing Kwong Sidney

Singapore General Hospital, Department of Nuclear Med and PET, Outram Road, Singapore 169608, Singapore

Sunil Kumar Udumala

Singapore General Hospital, Radiological Sciences Academic Clinical Program, Outram Road, Singapore 169608, Singapore

J. Biomed. Opt. 21(12), 126002 (Dec 01, 2016). doi:10.1117/1.JBO.21.12.126002
History: Received July 26, 2016; Accepted November 9, 2016
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Abstract.  Noninvasive and nonradioactive imaging modality to track and image apoptosis during chemotherapy of triple negative breast cancer is much needed for an effective treatment plan. Phosphatidylserine (PS) is a biomarker transiently exposed on the outer surface of the cells during apoptosis. Its externalization occurs within a few hours of an apoptotic stimulus by a chemotherapy drug and leads to presentation of millions of phospholipid molecules per apoptotic cell on the cell surface. This makes PS an abundant and accessible target for apoptosis imaging. In the current work, we show that PS monoclonal antibody tagged with indocyanine green (ICG) can help to track and image apoptosis using multispectral optoacoustic tomography in vivo. When compared to saline control, the doxorubicin treated group showed a significant increase in uptake of ICG-PS monoclonal antibody in triple negative breast tumor xenografted in NCr nude female mice. Day 5 posttreatment had the highest optoacoustic signal in the tumor region, indicating maximum apoptosis and the tumor subsequently shrank. Since multispectral optoacoustic imaging does not involve the use of radioactivity, the longer the circulatory time of the PS antibody can be exploited to monitor apoptosis over a period of time without multiple injections of commonly used imaging probes such as Tc-99m Annexin V or F-18 ML10. The proposed apoptosis imaging technique involving multispectral optoacoustic tomography, monoclonal antibody, and near-infrared absorbing fluorescent marker can be an effective tool for imaging apoptosis and treatment planning.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Ravi Kumar Kannadorai ; Sunil Kumar Udumala and Yu Wing Kwong Sidney
"Noninvasive in vivo multispectral optoacoustic imaging of apoptosis in triple negative breast cancer using indocyanine green conjugated phosphatidylserine monoclonal antibody", J. Biomed. Opt. 21(12), 126002 (Dec 01, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.12.126002


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