Research Papers: Sensing

Quantitative optical coherence microscopy for the in situ investigation of the biofilm

[+] Author Affiliations
Ratheesh Kumar Meleppat, Seah Leong Keey, Murukeshan Vadakke Matham

Nanyang Technological University, Centre for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, 50 Nanyang Avenue, Singapore 639798, Singapore

Christopher Shearwood

Nanyang Technological University, Biological Process Laboratory, School of Mechanical and Aerospace Engineering, 50 Nanyang Avenue, Singapore 639798, Singapore

J. Biomed. Opt. 21(12), 127002 (Dec 09, 2016). doi:10.1117/1.JBO.21.12.127002
History: Received August 27, 2016; Accepted November 17, 2016
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Abstract.  This paper explores the potential of optical coherence microscopy (OCM) for the in situ monitoring of biofilm growth. The quantitative imaging of the early developmental biology of a representative biofilm, Klebsiella pneumonia (KP-1), was performed using a swept source-based Fourier domain OCM system. The growth dynamics of the KP-1 biofilms and their transient response under perturbation was investigated using the enface visualization of microcolonies and their spatial localization. Furthermore, the optical density (OD) and planar density of the biofilms are calculated using an OCM technique and compared with OD and colony forming units measured using standard procedures via the sampling of the flow-cell effluent.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Ratheesh Kumar Meleppat ; Christopher Shearwood ; Seah Leong Keey and Murukeshan Vadakke Matham
"Quantitative optical coherence microscopy for the in situ investigation of the biofilm", J. Biomed. Opt. 21(12), 127002 (Dec 09, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.12.127002


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