Research Papers: Sensing

Fast spectral surface plasmon resonance imaging sensor for real-time high-throughput detection of biomolecular interactions

[+] Author Affiliations
Kaiqiang Chen, Youjun Zeng, Lei Wang, Xuejin Li, Junle Qu, Yonghong Shao

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen 518060, China

Dayong Gu, Jianan He

Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518033, China

Shu-Yuen Wu, Ho-Pui Ho

Chinese University of Hong Kong, Department of Electronic Engineering, Shatin, NT, Hong Kong

Bruce Zhi Gao

Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634, United States

J. Biomed. Opt. 21(12), 127003 (Dec 09, 2016). doi:10.1117/1.JBO.21.12.127003
History: Received September 3, 2016; Accepted November 18, 2016
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Abstract.  A fast surface plasmon resonance (SPR) imaging biosensor system based on wavelength interrogation using a liquid crystal tunable filter (LCTF) is presented. The system combines the merits of wide-dynamic detection range offered by the spectral approach and multiplexed high-throughput data collection with a two-dimensional (2-D) biosensor array. The key feature of the reported scheme is a feedback loop that drives the LCTF to achieve fast tracking of the SPR dip movement caused by the binding of target molecules to the sensor surface. Experimental results show that the system is capable of completing an SPR dip measurement within 4 s. Based on using a spectral window of about 100 nm, the experimental dynamic detection range and refractive index resolution are 4.63×102  RIU and 5.87×106  RIU, respectively. As also demonstrated herein using 2-D microsensor arrays, among the spectral SPR sensors, the reported system is most suitable for multiplexed label-free biosensing applications.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Kaiqiang Chen ; Youjun Zeng ; Lei Wang ; Dayong Gu ; Jianan He, et al.
"Fast spectral surface plasmon resonance imaging sensor for real-time high-throughput detection of biomolecular interactions", J. Biomed. Opt. 21(12), 127003 (Dec 09, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.12.127003


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