Research Papers: General

Feasibility study: protein denaturation and coagulation monitoring with speckle variance optical coherence tomography

[+] Author Affiliations
Changho Lee, Gyeongwoo Cheon, Jin U. Kang

Johns Hopkins University, Department of Electrical and Computer Engineering, 3400 North Charles Street, Baltimore, Maryland 21218, United States

Do-Hyun Kim

U.S. Food and Drug Administration, Center for Devices and Radiological Health, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993, United States

J. Biomed. Opt. 21(12), 125004 (Dec 10, 2016). doi:10.1117/1.JBO.21.12.125004
History: Received August 31, 2016; Accepted November 14, 2016
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Abstract.  We performed the feasibility study using speckle variance optical coherence tomography (SvOCT) to monitor the thermally induced protein denaturation and coagulation process as a function of temperature and depth. SvOCT provided the depth-resolved image of protein denaturation and coagulation with microscale resolution. This study was conducted using egg white. During the heating process, as the temperature increased, increases in the speckle variance signal was observed as the egg white proteins coagulated. Additionally, by calculating the cross-correlation coefficient in specific areas, denaturized egg white conditions were successfully estimated. These results indicate that SvOCT could be used to monitor the denaturation process of various proteins.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Changho Lee ; Gyeongwoo Cheon ; Do-Hyun Kim and Jin U. Kang
"Feasibility study: protein denaturation and coagulation monitoring with speckle variance optical coherence tomography", J. Biomed. Opt. 21(12), 125004 (Dec 10, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.12.125004


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