Research Papers: Imaging

Quantitative evaluation of atherosclerotic plaques using cross-polarization optical coherence tomography, nonlinear, and atomic force microscopy

[+] Author Affiliations
Ekaterina V. Gubarkova, Elena B. Kiseleva, Lidia B. Timofeeva, Felix I. Feldchtein, Natalia D. Gladkova

Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603950, Russia

Mikhail Yu. Kirillin, Alexander A. Moiseev, Gregory V. Gelikonov, Ilya I. Fiks

Institute of Applied Physics RAS, 46 Ulyanov Street, Nizhny Novgorod 603950, Russia

Varvara V. Dudenkova

Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603950, Russia

N.I. Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, Nizhny Novgorod 603950, Russia

Peter S. Timashev

Institute of Photonic Technologies, Research Center of Crystallography and Photonics RAS, 2 Pionerskaya Street, Troitsk, Moscow 142190, Russia

I.M. Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya Street, Moscow 119991, Russia

Svetlana L. Kotova, Galina V. Belkova, Anna B. Solovieva

N.N. Semenov Institute of Chemical Physics, 4 Kosygin Street, Moscow 119991, Russia

J. Biomed. Opt. 21(12), 126010 (Dec 20, 2016). doi:10.1117/1.JBO.21.12.126010
History: Received August 16, 2016; Accepted November 28, 2016
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Abstract.  A combination of approaches to the image analysis in cross-polarization optical coherence tomography (CP OCT) and high-resolution imaging by nonlinear microscopy and atomic force microscopy (AFM) at the different stages of atherosclerotic plaque development is studied. This combination allowed us to qualitatively and quantitatively assess the disorganization of collagen in the atherosclerotic arterial tissue (reduction and increase of CP backscatter), at the fiber (change of the geometric distribution of fibers in the second-harmonic generation microscopy images) and fibrillar (violation of packing and different nature of a basket-weave network of fibrils in the AFM images) organization levels. The calculated CP channel-related parameters are shown to have a statistically significant difference between stable and unstable (also called vulnerable) plaques, and hence, CP OCT could be a potentially powerful, minimally invasive method for vulnerable plaques detection.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Ekaterina V. Gubarkova ; Mikhail Yu. Kirillin ; Varvara V. Dudenkova ; Peter S. Timashev ; Svetlana L. Kotova, et al.
"Quantitative evaluation of atherosclerotic plaques using cross-polarization optical coherence tomography, nonlinear, and atomic force microscopy", J. Biomed. Opt. 21(12), 126010 (Dec 20, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.12.126010


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