The depth reconstruction in a setup closer to possible applications is demonstrated with measurements at a scattering phantom. The principles of the production and characterization of the optical properties of similar phantoms are described by Krauter et al.25 The phantom consists of a scattering matrix material with a scattering coefficient of and a refractive index of with inclusions of rhodamine 6G with sizes in the range of 10 to . The fluorescent inclusions are located in randomly distributed positions within the sample. The mean distance between the inclusions is adjusted, so only one fluorescent inclusion is placed in the field of view. To systematically investigate the depth reconstruction over a large measurement range, a single fluorescent inclusion in the phantom has been selected and has been positioned in different vertical positions. Hence, the distance to the focus of the microscope objective could be precisely controlled. Figure 6 shows the results of the phase shifting procedure for a set of different distances to the focus. In spite of the scattering, clear and well-evaluable interference patterns can be observed, and the data visualize the relation between defocusing and decreasing fringe density. The results show a similar signal quality as in Fig. 2, which underlines the efficiency of the phase shifting procedure in separating the interference signal from the scattering background. To quantify the signal quality, Fig. 7 shows one of the recorded frames and the fringe contrast obtained during the phase shifting procedure and characterizes the quality of the interferometric signal. In spite of the clearly visible noisy scattering background, a contrast in the range of 15% to 20% can be obtained over the full field of view with local perturbations due to impurities in the setup and inhomogeneities in the sample. The knowledge of the fringe contrast can be exploited to locally mask out regions of the image with poor signal quality. Again, the results are in good qualitative agreement with previous simulations,24 which have also shown that a sufficient contrast survives even for penetration depths of . Due to the increased fringe spacing for larger depths, an effect of averaging out of scattering components has been found, which leads to even decreased noise levels.