Special Section on Advanced Laser Technologies for Biophotonics

Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography

[+] Author Affiliations
Ruchire Eranga Wijesinghe, Mansik Jeon, Jeehyun Kim

Kyungpook National University, College of IT Engineering, School of Electronics Engineering, 80, Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea

Seung-Yeol Lee, Hee-Young Jung

Kyungpook National University, School of Applied Biosciences, 80, Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea

Pilun Kim

Oz-tec, Institute of Optics Image Diagnostic Technology, no 901, IT Convergence Industrial Building, 47, Gyeongdae-ro, 17-gil, Buk-gu, Daegu 41566, Republic of Korea

J. Biomed. Opt. 22(9), 091502 (Jan 05, 2017). doi:10.1117/1.JBO.22.9.091502
History: Received September 21, 2016; Accepted November 10, 2016
Text Size: A A A

Abstract.  Seed germination rate differs based on chemical treatments, and nondestructive measurements of germination rate have become an essential requirement in the field of agriculture. Seed scientists and other biologists are interested in optical sensing technologies-based biological discoveries due to nondestructive detection capability. Optical coherence tomography (OCT) has recently emerged as a powerful method for biological and plant material discoveries. We report an extended application of OCT by monitoring the germination rate acceleration of chemically primed seeds. To validate the versatility of the method, Capsicum annum seeds were primed using three chemical compounds: sterile distilled water (SDW), butandiol, and 1-hexadecene. Monitoring was performed using a 1310-nm swept source OCT system. The results confirmed more rapid morphological variations in the seeds treated with 1-hexadecene medium than the seeds treated with SDW and butandiol within 8 consecutive days. In addition, fresh weight measurements (gold standard) of seeds were monitored for 15 days, and the obtained results were correlated with the OCT results. Thus, such a method can be used in various agricultural fields, and OCT shows potential as a rigorous sensing method for selecting the optimal plant growth-promoting chemical compounds rapidly, when compared with the gold standard methods.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Ruchire Eranga Wijesinghe ; Seung-Yeol Lee ; Pilun Kim ; Hee-Young Jung ; Mansik Jeon, et al.
"Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography", J. Biomed. Opt. 22(9), 091502 (Jan 05, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.9.091502


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.