Research Papers: Imaging

Benchmarking of fluorescence cameras through the use of a composite phantom

[+] Author Affiliations
Dimitris Gorpas, Maximilian Koch, Maria Anastasopoulou, Vasilis Ntziachristos

Technical University Munich, Chair for Biological Imaging, Arcisstrasse 21, Munich D-80333, Germany

Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstrasse 1, Neuherberg D-85764, Germany

Uwe Klemm

Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstrasse 1, Neuherberg D-85764, Germany

J. Biomed. Opt. 22(1), 016009 (Jan 12, 2017). doi:10.1117/1.JBO.22.1.016009
History: Received September 27, 2016; Accepted December 23, 2016
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Abstract.  Fluorescence molecular imaging (FMI) has shown potential to detect and delineate cancer during surgery or diagnostic endoscopy. Recent progress on imaging systems has allowed sensitive detection of fluorescent agents even in video rate mode. However, lack of standardization in fluorescence imaging challenges the clinical application of FMI, since the use of different systems may lead to different results from a given study, even when using the same fluorescent agent. In this work, we investigate the use of a composite fluorescence phantom, employed as an FMI standard, to offer a comprehensive method for validation and standardization of the performance of different imaging systems. To exclude user interaction, all phantom features are automatically extracted from the acquired epi-illumination color and fluorescence images, using appropriately constructed templates. These features are then employed to characterize the performance and compare different cameras to each other. The proposed method could serve as a framework toward the calibration and benchmarking of FMI systems, to facilitate their clinical translation.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Dimitris Gorpas ; Maximilian Koch ; Maria Anastasopoulou ; Uwe Klemm and Vasilis Ntziachristos
"Benchmarking of fluorescence cameras through the use of a composite phantom", J. Biomed. Opt. 22(1), 016009 (Jan 12, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.1.016009


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