Research Papers: Imaging

Impact of temporal resolution on estimating capillary RBC-flux with optical coherence tomography

[+] Author Affiliations
Baoqiang Li, Buyin Fu, Sava Sakadžić, David A. Boas

Massachusetts General Hospital/Harvard Medical School, Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, Massachusetts 02129, United States

Hui Wang

Massachusetts General Hospital/Harvard Medical School, Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, Massachusetts 02129, United States

Massachusetts General Hospital/Harvard Medical School, Laboratory for Computational Neuroimaging, Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, Massachusetts 02129, United States

Ruopeng Wang

Massachusetts General Hospital/Harvard Medical School, Laboratory for Computational Neuroimaging, Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, Massachusetts 02129, United States

J. Biomed. Opt. 22(1), 016014 (Jan 26, 2017). doi:10.1117/1.JBO.22.1.016014
History: Received October 29, 2016; Accepted January 9, 2017
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Abstract.  Optical coherence tomography (OCT) has been used to measure capillary red blood cell (RBC) flux. However, one important technical issue is that the accuracy of this method is subject to the temporal resolution (Δt) of the repeated RBC-passage B-scans. A ceiling effect arises due to an insufficient Δt limiting the maximum RBC-flux that can be measured. In this letter, we first present simulations demonstrating that Δt=1.5  ms permits measuring RBC-flux up to 150  RBCs/s with an underestimation of 9%. The simulations further show that measurements with Δt=3 and 4.5 ms provide relatively less accurate estimates for typical physiological fluxes. We provide experimental data confirming the simulation results showing that reduced temporal resolution (i.e., a longer Δt) results in an underestimation of mean flux and compresses the distribution of measured fluxes, which potentially confounds physiological interpretation of the results. The results also apply to RBC-passage measurements made with confocal and two-photon microscopy for estimating capillary RBC-flux.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Baoqiang Li ; Hui Wang ; Buyin Fu ; Ruopeng Wang ; Sava Sakadžić, et al.
"Impact of temporal resolution on estimating capillary RBC-flux with optical coherence tomography", J. Biomed. Opt. 22(1), 016014 (Jan 26, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.1.016014


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