Research Papers: Sensing

Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip

[+] Author Affiliations
Martín A. Toderi

Óptica Aplicada a la Biología, IFIR (CONICET-UNR), Rosario, Santa Fe, Argentina

Horacio V. Castellini

Facultad de Ciencias Exactas Ingeniería y Agrimensura, Departamento de Física, UNR, Rosario, Santa Fe, Argentina

Bibiana D. Riquelme

Óptica Aplicada a la Biología, IFIR (CONICET-UNR), Rosario, Santa Fe, Argentina

Facultad de Cs. Bioquímicas y Farmacéuticas, Área Física, UNR, Rosario, Santa Fe, Argentina

J. Biomed. Opt. 22(1), 017003 (Jan 31, 2017). doi:10.1117/1.JBO.22.1.017003
History: Received November 3, 2016; Accepted January 4, 2017
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Abstract.  The study of red blood cell (RBC) aggregation is of great interest because of its implications for human health. Altered RBC aggregation can lead to microcirculatory problems as in vascular pathologies, such as hypertension and diabetes, due to a decrease in the erythrocyte surface electric charge and an increase in the ligands present in plasma. The process of erythrocyte aggregation was studied in stasis situation (free shear stresses), using an optical chip based on the laser transmission technique. Kinetic curves of erythrocyte aggregation under different conditions were obtained, allowing evaluation and characterization of this process. Two main characteristics of blood that influence erythrocyte aggregation were analyzed: the erythrocyte surface anionic charge (EAC) after digestion with the enzyme trypsin and plasmatic protein concentration in suspension medium using plasma dissolutions in physiological saline with human albumin. A theoretical approach was evaluated to obtain aggregation and disaggregation ratios by syllectograms data fitting. Sensible parameters (Amp100, t1\2) regarding a reduced erythrocyte EAC were determined, and other parameters (AI, M-Index) resulted that are representative of a variation in the plasmatic protein content of the suspension medium. These results are very useful for further applications in biomedicine.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Topics

Blood ; Lasers ; Plasmas ; Proteins

Citation

Martín A. Toderi ; Horacio V. Castellini and Bibiana D. Riquelme
"Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip", J. Biomed. Opt. 22(1), 017003 (Jan 31, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.1.017003


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