Research Papers: Sensing

Dual-modality optical biopsy of glioblastomas multiforme with diffuse reflectance and fluorescence: ex vivo retrieval of optical properties

[+] Author Affiliations
Vinh Nguyen Du Le

McMaster University, Radiation Sciences Graduate Program, Hamilton, Ontario, Canada

John Provias

McMaster University, Department of Anatomical Pathology, Hamilton, Ontario, Canada

Naresh Murty

McMaster University, Department of Surgery, Hamilton, Ontario, Canada

Michael S. Patterson

Juravinski Cancer Centre, Hamilton, Ontario, Canada

Zhaojun Nie

McMaster University, School of Biomedical Engineering, Hamilton, Ontario, Canada

Joseph E. Hayward, Thomas J. Farrell

Juravinski Cancer Centre, Hamilton, Ontario, Canada

McMaster University, School of Interdisciplinary Science, Hamilton, Ontario, Canada

William McMillan

Juravinski Cancer Centre, Hamilton, Ontario, Canada

McMaster University, Department of Oncology, Hamilton, Ontario, Canada

Wenbin Zhang

Shanghai Jiaotong University Medical School, Shanghai 9th People’s Hospital, Shanghai, China

Qiyin Fang

McMaster University, School of Biomedical Engineering, Hamilton, Ontario, Canada

McMaster University, Department of Engineering Physics, Hamilton, Ontario, Canada

J. Biomed. Opt. 22(2), 027002 (Feb 03, 2017). doi:10.1117/1.JBO.22.2.027002
History: Received July 7, 2016; Accepted January 12, 2017
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Abstract.  Glioma itself accounts for 80% of all malignant primary brain tumors, and glioblastoma multiforme (GBM) accounts for 55% of such tumors. Diffuse reflectance and fluorescence spectroscopy have the potential to discriminate healthy tissues from abnormal tissues and therefore are promising noninvasive methods for improving the accuracy of brain tissue resection. Optical properties were retrieved using an experimentally evaluated inverse solution. On average, the scattering coefficient is 2.4 times higher in GBM than in low grade glioma (LGG), and the absorption coefficient is 48% higher. In addition, the ratio of fluorescence to diffuse reflectance at the emission peak of 460 nm is 2.6 times higher for LGG while reflectance at 650 nm is 2.7 times higher for GBM. The results reported also show that the combination of diffuse reflectance and fluorescence spectroscopy could achieve sensitivity of 100% and specificity of 90% in discriminating GBM from LGG during ex vivo measurements of 22 sites from seven glioma specimens. Therefore, the current technique might be a promising tool for aiding neurosurgeons in determining the extent of surgical resection of glioma and, thus, improving intraoperative tumor identification for guiding surgical intervention.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Vinh Nguyen Du Le ; John Provias ; Naresh Murty ; Michael S. Patterson ; Zhaojun Nie, et al.
"Dual-modality optical biopsy of glioblastomas multiforme with diffuse reflectance and fluorescence: ex vivo retrieval of optical properties", J. Biomed. Opt. 22(2), 027002 (Feb 03, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.2.027002


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