Research Papers: Imaging

Motion characterization scheme to minimize motion artifacts in intravital microscopy

[+] Author Affiliations
Sungon Lee

Hanyang University, School of Electrical Engineering, Ansan, Republic of Korea

Gabriel Courties, Matthias Nahrendorf, Ralph Weissleder, Claudio Vinegoni

Richard B. Simches Research Center, Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States

J. Biomed. Opt. 22(3), 036005 (Mar 02, 2017). doi:10.1117/1.JBO.22.3.036005
History: Received July 1, 2016; Accepted February 13, 2017
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Abstract.  Respiratory- and cardiac-induced motion artifacts pose a major challenge for in vivo optical imaging, limiting the temporal and spatial imaging resolution in fluorescence laser scanning microscopy. Here, we present an imaging platform developed for in vivo characterization of physiologically induced axial motion. The motion characterization system can be straightforwardly implemented on any conventional laser scanning microscope and can be used to evaluate the effectiveness of different motion stabilization schemes. This method is particularly useful to improve the design of novel tissue stabilizers and to facilitate stabilizer positioning in real time, therefore facilitating optimal tissue immobilization and minimizing motion induced artifacts.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Sungon Lee ; Gabriel Courties ; Matthias Nahrendorf ; Ralph Weissleder and Claudio Vinegoni
"Motion characterization scheme to minimize motion artifacts in intravital microscopy", J. Biomed. Opt. 22(3), 036005 (Mar 02, 2017). ; http://dx.doi.org/10.1117/1.JBO.22.3.036005


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